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              Research
4.
The influence of age, sex and altitude on the morphometry of red blood cells in 
bovines - Nezar Adili, Mohamed Melizi and Omar Bennoune
  Vet World. 2013; 6(8): 476-478
              
               
              
              doi: 
              10.5455/vetworld.2013.476-478
               
              
  
  
   
  
              
               
               
               
              
              
              Abstract
              
              
  
  Aim: This study was conducted on 
  local bovine breed (black and white breed) in the east and the south of 
  Algeria in order to reveal the influence of age, sex and altitude on the 
  morphometry of red blood cells. Materials and Methods: The animals were 
  divided into three equal groups at different altitudes while the animals of 
  each group include adult males, adult females, young males, young females; and 
  live at the same altitude in the following regions: region A, Wilaya of Batna; 
  region B, Wilaya of Biskra; and region C, in the Wilaya of El-Oued. Blood 
  samples were collected from the jugular vein and blood smears were immediately 
  performed. The smears were stained according to the May-Grundwald-Giemsa 
  method. The morphometric study was achieved using with a graduted ocular and a 
  micrometric slide with an immersion optic microscope at a grossissement of 
  100. Statistical analysis was undertaken using the T student test. 
  
   Results: 
  The results showed a significant influence of age on the morphometry of red 
  blood cells, the young bovine's red blood cells are significantly larger than 
  the adults erythrocytes in the three regions. Indeed, sex have a significant 
  influence on the size of red blood cells, the erythrocytes of males are 
  significantly larger than the females erythrocytes in the three region. 
  However, concerning the altitude, the study showed a significant difference 
  between the Wilaya of Batna group and the two other groups, the bovine's red 
  blood cells of Batna Wilaya are significantly larger than the bovine's red 
  blood cells of Biskra and El-Oued groups. 
  
   Conclusion: Therefore, age, 
  sex and altitude have profound effect on the morphometry of red blood cells 
  and careful attention must be observed in studying and interpretation of 
  anemic syndromes. Keywords: Bovines, Black and White, Age, Sex, 
  Altitude, Morphometry.